Anne S. Robinson
- Director
- Associate Chair
- Full Professor
Education
- Doctorate - 1994 University of Illinois at Urbana-Champaign
- Masters - 1989 The Johns Hopkins University
- Bachelors - 1988 The Johns Hopkins University
"The Robinson laboratory is interested in understanding the fundamental interactions between molecules, both in isolation and in the complex environment of the cell. We use our growing understanding to design proteins with more robust or novel properties and to engineer cellular systems for improved production or drug screening applications. To this end, we are investigating the determinants of protein folding and misfolding, on both an atomic and molecular level. We have developed several novel approaches to inhibiting protein misfolding and aggregation. Additionally, we are designing cellular systems for optimal expression of membrane proteins, and antibodies.Our approach uses techniques in molecular biology, genetic engineering, and biophysical chemistry to identify and study macromolecules at both an atomic and cellular level. Mechanistic modeling guides us in experimental design and analysis. We use both prokaryotic, or bacterial, systems as well as eukaryotic systems, such as yeast or mammalian cells. A major goal of this research is to establish a set of cellular systems that could express any protein of interest. The research in the laboratory has focus areas in protein stability, expression, and aggregation for biotechnology and biomedical applications."
Selected Publications
For a complete listing of publications, please view the Full CV.
- Xu, P. and Robinson, A.S., "Decreased secretion and unfolded protein response up-regulation are correlated with intracellular retention for single-chain antibody variants produced in yeast", Biotech & Bioeng (2009)
- Spatara ML, Roberts CJ, Robinson AS, "Kinetic folding studies of the P22 tailspike beta-helix domain reveal multiple unfolded states", Biophys Chem (2009)
- Webber T, Gurung S, Saul J, Baker T, Spatara M, Freyer M, Robinson AS, Gage MJ, "The C-terminus of the P22 tailspike protein acts as an independent oligomerization domain for monomeric proteins", Biochem J (2009)
- McCusker, E., and Robinson, A.S., "Refolding of G protein ? subunits from inclusion bodies expressed.", Escherichia coli, Protein Exp. Purif. 58(2), 342, (2008)
- Russell, T.W.F., Robinson, A.S., and Wagner, N.J., "", (2008)
- Hildebrandt, S., D. Raden, L. Petzold, A.S. Robinson, and F.J. Doyle III, "A top-down approach to mechanistic biological modeling: application to the single-chain antibody folding pathway", Biophysical Journal 95(8), 3535, (2008)
- O'Malley, M., Lazarova, T., Britton, Z.T., and Robinson, A.S., "High-level expression in Saccharomyces cerevisiae enables isolation and spectroscopic characterization of functional human adenosine A?a receptor.", J. Struct Biol. 159(2), 166, (2007)
- Powers, S.L., Robinson, C.R., and Robinson, A.S., "Denaturation of an Extremely Stable Hyperthermophilic Protein Occurs via a Dimeric Intermediate", Extremophiles 11 (1), 179, (2007)
- Fama, M.C., Raden, D., Zacchi, N., Lemos, D.R., Robinson, A.S. and Silberstein, S., "The Saccharomyces cerevisiae YFR041C/ERJ5 gene encoding a type 1 membrane protein with a J domainis required to preserve the folding capacity of the endoplasmic reticulum.", Biochim Biophys Acta 1773 (2), 232, (2007)
- Bane, S.E., Velasquez, J.E., and A.S. Robinson, "Expression and Purification of Inactive G-protein coupled receptors.", E. coli, Protein Expression and Purification 52 (2), 348, (2007)
Patents
- Use of Hydrostatic Pressure to Inhibit and Reverse Protein Aggregation and Facilitate Protein Refolding. (Pending 10/99) (60/161,035): Robinson, A.S., D.Foguel, J.L. Silva, C., 1999
- Methods for Increasing Secretion of Overexpressed Proteins. (5,773,245): Robinson, A.S. and K.D. Wittrup, 1998
- Proteolytic Enzymes from Hyperthermophilic Bacteria and Processes for Their Production. (5,242,817): Kelly, R.M., A.K.S. Robinson, I.I. Blumentals, S.H. Brown, and C.B. Anfinsen, 1993
Selected Awards
For a complete listing of awards, please view the Full CV.
- NSF Presidential Early Career Award in Science and Engineering: 2000
- DuPont Young Professor: 2000 - 2003
- NIH Postdoctoral Fellowship: 1996 - 1997
- Clare Booth Luce Graduate Fellowship: 1992 - 1994
- Department of Defense Fellowship ((NDSEG): 1989 - 1992)
- NSF Creativity Award for Scientists and Engineers: 1988 - 1989
- Tau Beta Pi Fellowship: 1988 - 1989
- Beneficial Hodson Scholarship (Johns Hopkins University: 1984 - 1988)
- National Academy of Engineering, Sixth Annual Frontiers in Engineering (National Academy of Engineering)
- Outstanding Junior Faculty Member (College of Engineering, University of Delaware)
